Sheriff Provides Major DNA Update In Nancy Guthrie Investigation

A key piece of forensic evidence in the disappearance of Nancy Guthrie has not produced the breakthrough investigators were hoping for.

Pima County Sheriff Chris Nanos revealed Tuesday that DNA recovered from a glove found roughly two miles from Guthrie’s home was submitted to the FBI’s national database but did not return any matches.

The unknown male DNA profile was entered into the FBI’s Combined DNA Index System, known as CODIS, but “had no hits,” Nanos told Fox News chief correspondent Jonathan Hunt in an interview. The FBI later confirmed the development to Fox News Digital.

“We’re hopeful that we’re always getting closer, but the news now, I think, is we had heard this morning that, of course, the DNA on the glove that was found two miles away was submitted for CODIS,” Nanos said. “And I just heard that, CODIS had no hits.”

Investigators previously said forensic testing produced a male DNA profile from the glove that is believed to be linked to the masked individual seen on surveillance footage outside Guthrie’s Catalina Foothills home in the early morning hours of Feb. 1. That individual was observed tampering with a doorbell camera around the time the 84-year-old disappeared.

Authorities have also confirmed that the DNA found on the glove is distinct from genetic material collected inside the residence. Nanos said DNA recovered from inside the home was also run through CODIS and similarly produced no matches.

The case, which entered its third week Sunday, has drawn national attention in part because Nancy Guthrie is the mother of Savannah Guthrie, co-anchor of NBC’s “Today” show.

Investigators say blood matching Nancy Guthrie’s DNA was found around her property, intensifying concerns about foul play. However, no suspects or persons of interest have been publicly identified.

Earlier this month, Nanos formally cleared Guthrie’s family members as suspects, describing them as “victims” who have been fully cooperative with law enforcement throughout the investigation.

Authorities have released a description of the masked individual seen on video: a male between 5-foot-9 and 5-foot-10 with an average build. He was reportedly carrying a 25-liter Ozark Trail “Hiker Pack” backpack at the time.

While the absence of a CODIS match may slow the immediate path toward identifying a suspect, investigators stress that it does not close off potential leads. DNA profiles can generate matches later as new samples are added to the national database, and authorities are also exploring other investigative avenues.

The FBI and the Pima County Sheriff’s Department continue to urge anyone with information to come forward. The FBI has increased its reward to $100,000 for information leading to the location of Nancy Guthrie or the arrest and conviction of anyone involved in her disappearance.

Nanos indicated that additional law enforcement operations are expected in the coming days as the search intensifies. For now, officials say the investigation remains active and ongoing, with multiple forensic samples still under analysis.

“We’re hopeful,” Nanos said, signaling that despite the latest setback, investigators believe answers are still within reach.